{"id":535,"date":"2026-01-09T17:50:34","date_gmt":"2026-01-09T17:50:34","guid":{"rendered":"https:\/\/microlab.tn\/?post_type=product&#038;p=535"},"modified":"2026-04-10T20:55:30","modified_gmt":"2026-04-10T20:55:30","slug":"bouillon-mi","status":"publish","type":"product","link":"https:\/\/microlab.tn\/index.php\/product\/bouillon-mi\/","title":{"rendered":"Bouillon MI"},"content":{"rendered":"<p><span style=\"font-weight: 300;\">Pour le d\u00e9nombrement d\u2019E. Coli, comptez toutes les colonies bleues \u00e0 la surface de la membrane sous lumi\u00e8re normale\/ambiante.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 300;\">Pour le d\u00e9nombrement des coliformes totaux, exposez chaque membrane \u00e0 la lumi\u00e8re ultraviolette \u00e0 ondes longues (366 nm) puis comptez toutes les colonies fluorescentes.<\/span><\/p>\n<p><span style=\"font-weight: 300;\">Les colonies bleues\/vertes fluorescentes ou bleues\/vertes avec bords fluorescents sont des <\/span><i><span style=\"font-weight: 300;\">E. coli<\/span><\/i><span style=\"font-weight: 300;\">. Les colonies bleues\/blanches fluorescentes correspondent aux coliformes totaux (autres que E. Coli). Ajoutez \u00e9galement les colonies bleues non fluorescentes au d\u00e9nombrement des coliformes totaux.<\/span><\/p>\n<p><span style=\"font-weight: 300;\">Les bouillons Microclar sont con\u00e7us pour une utilisation industrielle et sont disponibles soit en ampoule en plastique ou en verre.<\/span><\/p>\n<p><span style=\"font-weight: 300;\">Mode d\u2019emploi pour la m\u00e9thode de filtration sur membrane:<\/span><\/p>\n<p><span style=\"font-weight: 300;\">Ouvrez le flacon et r\u00e9partissez son contenu sur le tampon absorbant en cellulose \u00e0 l\u2019int\u00e9rieur de la bo\u00eete de Petri. \u00c0 l\u2019aide d\u2019une pince flamb\u00e9e, retirez la membrane de l\u2019unit\u00e9 de filtration et placez-la sur le tampon imbib\u00e9, en veillant \u00e0 ce qu\u2019aucune bulle d\u2019air ne soit emprisonn\u00e9e entre les deux. Couvrez la bo\u00eete. Placez-la dans un incubateur et incubez selon les exigences du milieu choisi. Pendant l\u2019incubation, les nutriments du milieu migrent par capillarit\u00e9 vers la surface de la membrane, nourrissent les micro-organismes et permettent le d\u00e9veloppement de colonies qui pourront ensuite \u00eatre d\u00e9nombr\u00e9es.<\/span><\/p>\n<p><b>Sp\u00e9cifications<\/b><\/p>\n<table>\n<tbody>\n<tr>\n<td><span style=\"font-weight: 300;\">Microorganismes<\/span><\/td>\n<td><span style=\"font-weight: 300;\">Coliformes totaux et E. Coli<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 300;\">Couleur<\/span><\/td>\n<td><span style=\"font-weight: 300;\">ambre clair<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 300;\">pH<\/span><\/td>\n<td><span style=\"font-weight: 300;\">7,0 \u00b1 0,2 pH \u00e0 25\u00b0C<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 300;\">Incubation recommand\u00e9e<\/span><\/td>\n<td><span style=\"font-weight: 300;\">Pendant 18 \u00e0 24 heures \u00e0 35 \u00b1 2 \u00b0C<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 300;\">Croissance<\/span><\/td>\n<td><span style=\"font-weight: 300;\">Sous lumi\u00e8re normale\/ambiante : colonies d\u2019E.Coli bleues.<\/span><\/p>\n<p><span style=\"font-weight: 300;\">Sous lumi\u00e8re UV : E. coli bleues\/vertes fluorescentes ou bleues\/vertes avec bords fluorescents. Les coliformes totaux sont bleues\/blanches fluorescentes.\u00a0<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 300;\">Quantit\u00e9<\/span><\/td>\n<td><span style=\"font-weight: 300;\">50x2mL\/Pk<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 300;\">Stockage<\/span><\/td>\n<td><span style=\"font-weight: 300;\">2\u00b0C to 40\u00b0C<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 300;\">Dur\u00e9e de conservation<\/span><\/td>\n<td><span style=\"font-weight: 300;\">Ampoule en plastique 12 mois\u00a0\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><br style=\"font-weight: 400;\" \/><br style=\"font-weight: 400;\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li aria-level=\"1\"><span style=\"font-weight: 300;\">D\u00e9tection des coliformes totaux et des E. Colis par la m\u00e9thode de filtration sur membrane.<\/span><\/li>\n<li aria-level=\"1\">Fabricant : <span style=\"font-weight: 300;\">Microclar<\/span><\/li>\n<li aria-level=\"1\">R\u00e9f\u00e9rence : 0<\/li>\n<\/ul>\n","protected":false},"featured_media":1100,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":""},"product_brand":[],"product_cat":[28,45],"product_tag":[],"class_list":["post-535","product","type-product","status-publish","has-post-thumbnail","product_cat-analyse-microbiologique-biochimique","product_cat-microbiologie-diagnostique","first","instock","shipping-taxable","product-type-simple"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/product\/535","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/comments?post=535"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/media\/1100"}],"wp:attachment":[{"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/media?parent=535"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/product_brand?post=535"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/product_cat?post=535"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/microlab.tn\/index.php\/wp-json\/wp\/v2\/product_tag?post=535"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}